73043-80-8

Basic information

• Product Name: 7-nitro-1-methyl-1H-benzo[d][1,3]oxazine-2,4-dione
• Synonyms: 7-nitro-1-methyl-1H-benzo[d][1,3]oxazine-2,4-dione;1-Methyl-7-nitro-1H-benzo[d][1,3]oxazine-2,4-dione;1-Methyl-7-nitroisatoic anhydride;1-METHYL-7-NITROISATOIC ANHYDRIDE(WX142523)
• CAS NO: 73043-80-8
• Molecular Formula: C₉H₆N₂O₅
• Molecular Weight: 222.15434
• Mol File: 73043-80-8.mol
• Article Data: 8

Chemical Properties

• Melting Point: 203-206 °C
• Boiling Point: 395.4±44.0 °C(Predicted)
• Appearance: /
• Density: 1.551±0.06 g/cm3(Predicted)
• Storage Temp.: 2-8°C
• Solubility: Chloroform (Slightly, Heated)
• PKA: -4.82±0.20(Predicted)
• Stability: Moisture Sensitive
• CAS DataBase Reference: 7-nitro-1-methyl-1H-benzo[d][1,3]oxazine-2,4-dione(CAS DataBase Reference)
• NIST Chemistry Reference: 7-nitro-1-methyl-1H-benzo[d][1,3]oxazine-2,4-dione(73043-80-8)
• EPA Substance Registry System: 7-nitro-1-methyl-1H-benzo[d][1,3]oxazine-2,4-dione(73043-80-8)

Safety Data

• Hazardous Substances Data: 73043-80-8(Hazardous Substances Data)

Usage

Description

7-nitro-1-methyl-1H-benzo[d][1,3]oxazine-2,4-dione, also known as 1-Methyl-7-nitroisatoic anhydride (1M7), is a chemical compound that serves as an in vivo SHAPE-MaP reagent for live cell RNA structure analysis at single nucleotide resolution.

Uses

Used in RNA Structure Analysis:
7-nitro-1-methyl-1H-benzo[d][1,3]oxazine-2,4-dione is used as a selective 2′-hydroxyl acylation reagent in the SHAPE (selective 2′-hydroxyl acylation analyzed by primer extension) method for live cell RNA structure analysis. It reacts with the 2′-hydroxyl group of RNA, providing valuable insights into RNA structure and interactions.
Used in Transcriptome Analysis:
When combined with mutational profiling (MaP), 7-nitro-1-methyl-1H-benzo[d][1,3]oxazine-2,4-dione enables quantitative nucleotide measurements for entire transcriptomes, deepening the understanding of RNA interactions and regions that may be exploited for the design of RNA-targeting small-molecule drugs.

Upstream product

• 541-41-3 chloroformic acid ethyl ester 
• 77-78-1 dimethyl sulfate 
• 619-17-0 4-Nitroanthranilic acid 
• 201230-82-2 carbon monoxide 
• 619-26-1 N-methyl-3-nitroaniline 
• 63480-10-4 4-nitroisatoic anhydride 
• 74-88-4 methyl iodide 

Downstream Products

• 49565-62-0 2-(methylamino)-4-nitro-benzoic acid 

Relevant articles and documents

A fast-acting reagent for accurate analysis of RNA secondary and tertiary structure by SHAPE chemistry

Selective 2′-hydroxyl acylation analyzed by primer extension (SHAPE) chemistry allows local nucleotide flexibility to be quantitatively assessed at single nucleotide resolution in any RNA. SHAPE chemistry exploits structure-based gating of the nucleophili

Doxorubicin-loaded polymeric micelle as well as preparation method and application thereof

The present invention discloses a doxorubicin-loaded polymeric micelle as well as a preparation method and application thereof. Novel amino evodiamine is adopted to react with polyethylene glycol, a micelle obtained by self-assembly is used as a carrier to load doxorubicin, a prepared mPEG-CO-NH-EVO micelle and a mPEG-CO-NH-EVO-OCH3 micelle have low toxicity to normal hepatocytes so that the two amphipathic polymeric micelles are used as carriers to load DOX to prepare DOX-loaded polymeric micelles, namely a mPEG-CO-NH-EVO/DOX micelle and a mPEG-CO-NH-EVO-OCH3/DOX micelle, and DOX enters hydrophobic shells of the micelles due to the dialysis effect; shapes of the micelles are represented by using a transmission electron microscope and a scanning electron microscope, and the drug loading efficiency and encapsulation efficiency of DOX are tested and calculated by using an ultraviolet spectrophotometer; and finally, evaluating the cytotoxicity by using a CCK-8 method. Shown by cytotoxicity tests, a DOX-loaded polymer has an effect on inhibiting breast cancer cells and particularly has greatly reduced toxicity to the normal hepatocytes.

Synthesis and antimicrobial activity of novel 4-Hydroxy-2-quinolone analogs

Alkyl quinolone has been proven to be a privileged scaffold in the antimicrobial drug discovery pipeline. In this study, a series of new 4-hydroxy-2-quinolinone analogs containing a long alkyl side chain at C-3 and a broad range of substituents on the C-6 and C-7 positions were synthesized. The antibacterial and antifungal activities of these analogs against Staphylococcus aureus, Escherichia coli, and Aspergillus flavus were investigated. The structure-activity relationship study revealed that the length of the alkyl chain, as well as the type of substituent, has a dramatic impact on the antimicrobial activities. Particularly, the brominated analogs 3j with a nonyl side chain exhibited exceptional antifungal activities against A. flavus (half maximal inhibitory concentration (IC50) = 1.05 μg/mL), which surpassed that of the amphotericin B used as a positive control. The antibacterial activity against S. aureus, although not as potent, showed a similar trend to the antifungal activity. The data suggest that the 4-hydroxy-2-quinolone is a promising framework for the further development of new antimicrobial agents, especially for antifungal treatment.