79259-44-2Relevant articles and documents
Cross-linked crystals of subtilisin: Versatile catalyst for organic synthesis
Wang, Yi-Fong,Yakovlevsky, Kirill,Zhang, Bailing,Margolin, Alexey L.
, p. 3488 - 3495 (2007/10/03)
Cross-linked enzyme crystals (CLECs) of subtilisin exhibit excellent activity in aqueous and various organic solvents. This catalyst is more stable than the native enzyme in both aqueous and mixed aqueous/organic solutions. Subtilisin-CLEC was shown to be a versatile catalyst. It was used for the syntheses of peptides and peptidomimetics, mild hydrolysis of amino acid and peptide amides, enantio- and regioselective reactions, and transesterifications.
α-Chymotrypsin-catalyzed Synthesis of Tripeptide Amides in an Aqueous-Organic Two-phase System
Kuhl, P.,Posselt, Siegrid,Jakubke, H.-D.
, p. 463 - 465 (2007/10/02)
α-Chymotrypsin catalyzes in a biphasic system containing an organic solvent and hydrogencarbonate buffer the reaction of Boc-Leu-Phe-OMe with X-NH2 (X=Leu, Met, Val) forming Boc-tripeptide amides.In the case of X=Leu the addition of ammoniumsulfate is advantageous and allows the use of carboxyl and amino component in equivalent amounts.By the same method Z-protected tripeptide amides can be prepared from Z-X-Phe-OMe (X=Ala, Leu, Val) and Leu-NH2 in good yields.In some case α-chymotrypsin-catalyzed peptide coupling is also possible, if the buffer solution is substituted for crystal water containing sodium carbonate.
