9014-25-9

Basic information

• Product Name: TRNA, BAKER'S YEAST
• Synonyms: RIBONUCLEIC ACID, TRANSFER FROM BAKERS*Y EAST;RIBONUCLEIC ACID TRANSFER TYPE XII FROM RABBIT LIVE;RIBONUCLEIC ACID TRANSFER TYPE X-SA*FROM BAKERS YEA;RIBONUCLEIC ACID, TRANSFER FROM RABBIT L IVER;RIBONUCLEIC ACID TRANSFER TYPE XXI*FROM ESCHERICHIA;RIBONUCLEIC ACID, TRANSFER TYPE XI*FROM BOVINE LIVE;RIBONUCLEIC ACID, TRANSFER-MOLECULAR*BIO LOGY GRADE;RIBONUCLEIC ACID TRANSFER TYPE X*FROM BA KERS YEAST
• CAS NO: 9014-25-9
• Molecular Weight: 0
• EINECS: 277-256-7
• Mol File: 9014-25-9.mol
• Article Data: 0

Chemical Properties

• Appearance: /lyophilized powder
• Storage Temp.: −20°C
• Solubility: H2O: 2 mg/mL, clear, colorless
• CAS DataBase Reference: TRNA, BAKER'S YEAST(CAS DataBase Reference)
• NIST Chemistry Reference: TRNA, BAKER'S YEAST(9014-25-9)
• EPA Substance Registry System: TRNA, BAKER'S YEAST(9014-25-9)

Safety Data

• Safety Statements: 22-24/25
• WGK Germany: 3
• F: 10-21
• Hazardous Substances Data: 9014-25-9(Hazardous Substances Data)

Usage

Description

Transfer RNA (tRNA), also known as BAKER'S YEAST, is a type of ribonucleic acid that is isolated from baker's yeast (S. cerevisiae) through phenol-chloroform extraction and ethanol precipitation. It is an approximately 80 nucleotides long RNA molecule with a cloverleaf shaped secondary structure and a tertiary L-shaped structure. tRNA plays a crucial role in protein synthesis by carrying specific amino acids to the ribosome during translation.

Uses

Used in Molecular Biology Research:
tRNA, BAKER'S YEAST is used as a starting material for the purification of specific amino acyl-tRNA species by methods such as liquid column chromatography or high-performance liquid chromatography (HPLC). This allows researchers to study the structure, function, and interactions of tRNA with other cellular components.
Used in Nucleic Acid Purification and Precipitation:
tRNA, BAKER'S YEAST is used as a carrier in nucleic acid purification and precipitation. It enhances the recovery of RNA from small numbers of cells, making it a valuable tool in various molecular biology applications.
Used in Reverse Transcriptase – Quantitative Polymerase Reaction (RT-qPCR) Assay:
tRNA, BAKER'S YEAST is used as a component of the carrier solution in RT-qPCR assays. It serves as a control to ensure accurate quantification of target nucleic acid sequences.
Used in In Situ Hybridization:
tRNA, BAKER'S YEAST is used as a component of prehybridization and hybridization buffer in single-label in situ hybridization. It helps to improve the specificity and sensitivity of the hybridization process, allowing for the detection of specific nucleic acid sequences within cells or tissue sections.

Biochem/physiol Actions

Transfer RNAs (tRNAs) play an important role in translation. It is responsible for the addition of amino acids to ribosome for peptide chain formation. The tRNA contains an anticodon region for mRNA base pairing and two attachment regions: for amino acid binding and tRNA synthetase recognition. The ribosomal RNA facilitates the movement of tRNA along the mRNA.