Full Length ArticleComparison of Diglycolic acid (cas 110-99-6) exposure to human proximal tubule cells in vitro and rat kidneys in vivo
-
Add time:08/24/2019 Source:sciencedirect.com
Diglycolic acid (cas 110-99-6) (DGA) is present in trace amounts in our food supply and is classified as an indirect food additive linked with the primary GRAS food additive carboxymethyl cellulose (CMC). Carboxymethyl starches are used as a filler/binder excipient in dietary supplement tablets and a thickening ingredient in many other processed foods. We sought to utilize the human proximal tubule HK-2 cell line as an in vitro cellular model system to evaluate its acute nephrotoxicity of DGA. We found that DGA was indeed toxic to HK-2 cells in all in vitro assays in our study, including a highly sensitive Luminex assay that measures levels of an in vitro biomarker of kidney-specific toxicity, Kidney Injury Molecule 1 (KIM-1). Interestingly, in vitro KIM-1 levels also correlated with in vivo KIM-1 levels in urine collected from rats treated with DGA by daily oral gavage. The use of in vitro and in vivo models towards understanding the effectiveness of an established in vitro system to predict in vivo outcomes would be particularly useful in rapidly screening compounds that are suspected to be unsafe to consumers. The merit of the HK-2 cell model in predicting human toxicity and accelerating the process of food toxicant screening would be especially important for regulatory purposes. Overall, our study not only revealed the value of HK-2 in vitro cell model for nephrotoxicity evaluation, but also uncovered some of the mechanistic aspects of the human proximal tubule injury that DGA may cause.
We also recommend Trading Suppliers and Manufacturers of Diglycolic acid (cas 110-99-6). Pls Click Website Link as below: cas 110-99-6 suppliers
Prev:Regular articleDiglycolic amic acid-modified E. coli as a biosorbent for the recovery of rare earth elements
Next:Diglycolic acid (cas 110-99-6) inhibits succinate dehydrogenase activity in human proximal tubule cells leading to mitochondrial dysfunction and cell death) - 【Back】【Close 】【Print】【Add to favorite 】
- Related Information
- The effect of ligand rigidity on the stability of europium(III) complexes with substituted Diglycolic acid (cas 110-99-6)s08/27/2019
- Influence of ether linkage on the enzymatic degradation of PBS copolymers: Comparative study on poly (butylene succinate-co-diethylene glycol succinate) and poly (butylene succinate-co-butylene Diglycolic acid (cas 110-99-6))08/26/2019
- Diglycolic acid (cas 110-99-6) inhibits succinate dehydrogenase activity in human proximal tubule cells leading to mitochondrial dysfunction and cell death08/25/2019
- Regular articleDiglycolic amic acid-modified E. coli as a biosorbent for the recovery of rare earth elements08/23/2019
- A novel sensor based on electrochemical polymerization of Diglycolic acid (cas 110-99-6) for determination of acetaminophen08/22/2019
- 28-day repeated dose response study of Diglycolic acid (cas 110-99-6): Renal and hepatic effects☆08/21/2019
- Diglycolic acid (cas 110-99-6) induces HepG2/C3A liver cell toxicity in vitro08/20/2019
-
Health and Chemical more >