Simultaneous determination of betaine and N,N-Dimethylglycine (cas 1118-68-9) in urine
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Add time:09/25/2019 Source:sciencedirect.com
A method is described for the determination of betaine and its metabolite N,N-Dimethylglycine (cas 1118-68-9) in human urine. The method involves a deamination step and a solid-phase extraction to isolate both substances from urine followed by high performance liquid chromatography (HPLC) separation on a 5-μm SCX column with UV absorbance detection. Limit of detection is about 0.9 μg for both substances. Recoveries were > 85%. The method appears to be suitable for monitoring betaine and its metabolite in urine of patients undergoing therapy with betaine.
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