1185-53-1 Usage
Description
2-Amino-2-(hydroxymethyl)-1,3-propanediol hydrochloride, also known as Tris hydrochloride, is a high-quality buffer component commonly used in various biological and biochemical applications. It is a white crystalline powder with a pKa of 8.08 at 25°C and a pH range of 7.0 to 9.0, which coincides with the physiological pH of most living organisms. Tris hydrochloride is often mixed with Tris base to provide a buffer with a pH between 7 and 9, offering adequate buffering capacity.
Uses
Used in Electrophoresis:
2-Amino-2-(hydroxymethyl)-1,3-propanediol hydrochloride is used as a buffer component in SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) for the separation of proteins based on their molecular weight. It helps maintain a stable pH environment during the electrophoresis process.
Used in Transgenic Mouse Generation:
2-Amino-2-(hydroxymethyl)-1,3-propanediol hydrochloride is used as a buffer component in the robust generation of transgenic mice, ensuring a stable pH environment for genetic manipulation and expression studies.
Used in Biological and Biochemical Applications:
2-Amino-2-(hydroxymethyl)-1,3-propanediol hydrochloride is used as a buffer component in various biological and biochemical applications, providing a stable pH environment that is compatible with biological fluids and suitable for a wide range of experiments.
Used in Protein Crystallization:
2-Amino-2-(hydroxymethyl)-1,3-propanediol hydrochloride is used as a buffer component for the crystallization of proteins at various pH values, facilitating the study of protein structures and functions.
Used in Custom Buffer Preparation:
2-Amino-2-(hydroxymethyl)-1,3-propanediol hydrochloride is used as a component in the preparation of custom running and loading buffers, often in combination with glycine and SDS, for various electrophoresis techniques.
Application Industry: Molecular Biology and Genetics
2-Amino-2-(hydroxymethyl)-1,3-propanediol hydrochloride is used as a buffer component for maintaining a stable pH environment in molecular biology and genetics research, facilitating various experimental procedures and analyses.
Application Industry: Biochemistry
2-Amino-2-(hydroxymethyl)-1,3-propanediol hydrochloride is used as a buffer component in biochemistry, ensuring a stable pH environment for enzyme assays, protein interactions, and other biochemical reactions.
Preparation
The preparation of TRIS hydrochloride is as follows:After reacting 2-amino-1,3-propanediol (2.50 g, 27.44 mmol) and formic acid (1.26 g, 27.44 mmol) in 50 ml of water at room temperature for 3 hours, water was distilled off under reduced pressure, A liquid was obtained. By washing the obtained liquid,An ammonium formate salt (3.76 g, 27.44 mmol) was obtained as a colorless transparent liquid.
Purification Methods
Crystallise the salt from 50% EtOH, then from 70% EtOH. TRIS-hydrochloride is also available commercially in a highly pure state. Otherwise, recrystallise it from 50% EtOH, then 70% EtOH, and dry it below 40o to avoid risk of decomposition. [Beilstein 4 H 304.]
Check Digit Verification of cas no
The CAS Registry Mumber 1185-53-1 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 1,1,8 and 5 respectively; the second part has 2 digits, 5 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 1185-53:
(6*1)+(5*1)+(4*8)+(3*5)+(2*5)+(1*3)=71
71 % 10 = 1
So 1185-53-1 is a valid CAS Registry Number.
InChI:InChI=1/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H/p-1
1185-53-1Relevant articles and documents
The hydrophilic ionic liquid at room temperature and its use (by machine translation)
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Paragraph 0099; 0100; 0237; 0254, (2018/10/24)
PROBLEM TO BE SOLVED: To provide a novel ionic liquid that has a cation structure apt to become liquid, and is highly hydrophilic, particularly highly water-soluble, and use thereof.SOLUTION: This invention provides a hydrophilic room-temperature ionic liquid including a cation and an anion, the cation being a quaternary ammonium cation of the formula (I), where Rs independently represent a 2-8C straight-chain or branched-chain polyhydroxyalkyl group having two or more hydroxy groups, Rs independently represent a hydrogen atom or a 1-5C straight-chain or branched-chain monohydroxyalkyl group, and n represents an integer of 1-4.
Phenoxybenzylamine derivatives as SSRls
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, (2008/06/13)
A compound of general formula (I) wherein R1 and R2 are H, C1-C6alkyl or (CH2)d(C3-C6cycloalkyl) wherein d=0, 1, 2 or 3; or R1 and R2 together with the nitrogen to which they are attached form an azetidine ring; Z or Y is —SR3 and the other Z or Y is halogen or —R3; wherein R3 is C1-C4 alkyl optionally substituted with fluorine; except that R3 is not CF3; or Z and Y are linked so that, together with the interconnecting atoms, Z and Y form a fused 5 to 7-membered carbocyclic or heterocyclic ring, and wherein when Z and Y form a heterocyclic ring, in addition to carbon atoms, the linkage contains one or two heteroatoms independently selected from oxygen, sulfur and nitrogen; R4 and R5, which may be the same or different, are: A—X, wherein A═—CH═CH— or —(CH2)p— where p is 0, 1 or 2; X is hydrogen, F, Cl, Br, I, CONR6R7, SO2NR6R7, SO2NHC(═O)R6, OH, C1-4alkoxy, NR8SO2R9, NO2, NR6R11, CN, CO2R10, CHO, SR10, S(O)R9 or SO2R10; or a 5- or 6-membered heterocyclic ring containing 1, 2 or 3 heteroatoms selected from N, S and O, optionally substituted independently by one or more R13; wherein R13 is hydroxy, C1-C4alkoxy, F, C1-C6alkyl, haloalkyl, haloalkoxy, —NH2, —NH(C1-C6alkyl) or —N(C1-C6alkyl)2.
Methods for protecting ZEA mays plants against pest damage
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, (2008/06/13)
According to the invention there is provided a method of controlling insect larvae comprising the steps of feeding said larvae an insecticidal amount of a transgenic Zea mays plant that expresses a polypeptide having the insect toxicity properties of Bacillus thuringiensis crystal protein.