1271731-41-9Relevant articles and documents
A Novel Organometallic ReI Complex with Favourable Properties for Bioimaging and Applicability in Solid-Phase Peptide Synthesis
Raszeja, Lukasz,Maghnouj, Abdelouahid,Hahn, Stephan,Metzler-Nolte, Nils
, p. 371 - 376 (2011)
Organometallic complexes possess great potential for imaging applications in biology, due to their kinetic stability and often favourable intrinsic properties. In this work we present a new class of ReI-tricarbonyl complexes with a substituted bis(phenanthridinylmethyl)amine (bpm) ligand. The complex Re(CO)3(R-bpm) could be conveniently prepared by microwave synthesis from [Re(CO)3(H2O)3]Br and a suitably substituted bis(phenanthridinylmethyl)amine (R-bpm). Complex 5, with R=CH2-CO2-CH3, was characterized by a single-crystal X-ray structure. Complex 6 (R=CH2-C6H4-CO2H) was used in solid-phase peptide synthesis (SPPS) to label the neurotensin(8-13) (NT) fragment N-terminally. The complexes show luminescence emission with large Stokes shifts (λex=350 nm, λem=570 nm). Cellular uptake and intracellular localization studies in several cell lines demonstrate the utility of the new Re(CO)3(R-bpm) complexes for fluorescence imaging and reveal significant differences between the simple methyl ester 5 and the NT bioconjugate 7. Imaging with metal complexes: The synthesis and characterization of a novel bis(phenanthridinylmethyl) (bpm) ligand and its rhenium tricarbonyl complexes are described. Moreover it is shown that the advantageous photophysical properties of these complexes could be exploited for application in cellular imaging experiments.
