188862-35-3Relevant articles and documents
Development of strategies for the site-specific in vivo incorporation of photoreactive amino acids: p-Azidophenylalanine, p-acetylphenylalanine and benzofuranylalanine
Behrens, Carsten,Nielsen, Jens N.,Fan, Xue-Jun,Doisy, Xavier,Kim, Ki-Hyun,Praetorius-Ibba, Mette,Nielsen, Peter E.,Ibba, Michael
, p. 9443 - 9449 (2000)
A major limitation of conventional site-directed mutagenesis is that substitutions are restricted to the 20 naturally occurring amino acids. While this problem can be circumvented in vitro to allow the site-specific incorporation of non-canonical amino acids, no similar in vivo methodologies yet exist. The main requirement for such a system is an aminoacyl-tRNA synthetase able to exclusively recognize a non-canonical amino acid and a suppressor tRNA. The engineering of such activities in aminoacyl-tRNA synthetases has proven to be problematic due to their high substrate specificity. Here we report progress towards the development of an antibody-based methodology to screen large mutant aminoacyl-tRNA synthetase libraries for specific recognition of the non-canonical photoreactive benzofuran amino acid [3-(5'-benzofuranyl)-alanine]. We also report the chemical synthesis and enantiomeric resolution of this amino acid. (C) 2000 Elsevier Science Ltd.
TRIAZOLOPYRIDAZINES AS TYROSINE KINASE MODULATORS
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Page/Page column 96, (2008/06/13)
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Piperidine derivative rennin inhibitors
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Page/Page column 18, (2010/02/08)
Disclosed are piperidine derivatives, their manufacture and use as inhibitors of renin.
